Plant growth promoting characterization of indigenous phosphate solubilizing rhizobacteria and effects on germination of some crops in Vietnam
Keywords:plant growth promoting activity, phosphate solubilizing bacteria, rhizosphere, seed, solubility index
Inorganic Phosphate Solubilizing Bacteria (PSB) are widely present in nature and have been successfully applied in fields in many countries. However, researches on indigenous PSB are still very limited in Vietnam. The objective of this study was to isolate and evaluate the growth promoting characteristics and effects on germination of seed of indigenous PSB for fertilizer production from PSB. Thirteen isolates of indigenous PSB were collected using selective isolation medium containing Ca3(PO4)2, of which eight added the ability to dissolve AlPO4 and five isolates added the ability to dissolve FePO4. Initial qualitative tests indicated that all thirteen PSB isolates were incapable of HCN and lipase production while other growth promoting activities including amylase, caseinase, cellulase, chitinase, pectinase, indole acetic acid, K solubility, Zn solubility, and N fixation varied according to PSB isolate. The collected PSB isolates had no effect on seed germination rate, root length, and hypocotyl length of plantules of soybean, rice, maize, cucumber, tomato, and chili by plate assay. This study had shown that PSB was also common in the rhizosphere of various crops in Vietnam. Therefore, isolation to enrich the indigenous PSB collection was essential for the screening of suitable PSB strains for subsequent fertilizer production.
Abdelsamei HA, Ibrahim EM, Sohaimy SA, Sad MA (2015). Effect of storage on the activity of the bacteriocin extracted from Lactobacillus acidophilus. Benha Veterinary Medical Journal 28(1):216-222.
Aliyat FZ, Maldani M, El Guilli M, Nassiri L, Ibijbijen J (2022). Phosphate-solubilizing bacteria isolated from phosphate solid sludge and their ability to solubilize three inorganic phosphate forms: calcium, iron, and aluminum phosphates. Microorganisms 2022(10):980. https://doi.org/10.3390/microorganisms10050980
Amaresan N, Patel P, Amin D (2022). Practical handbook on agricultural microbiology. Humana Press. New York.
Amy C, Avice J-C, Laval K, Bressan M (2022). Are native phosphate solubilizing bacteria a relevant alternative to mineral fertilizations for crops? Part I. When rhizobacteria meet plant P requirements. Rhizosphere 21(2022):100476. https://doi.org/10.1016/j.rhisph.2022.100476
Angshumanjana A, Dipjitdey A, Jayantabikashdey TN (2016). Selection of storage methods for maintenance of different stock cultures. International Journal of Current Microbiology and Applied Sciences 5(10):1097-1104. http://dx.doi.org/10.20546/ijcmas.2016.510.115
Bashan Y, Kamnev AA, de-Bashan LE (2013). Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure. Biology and Fertility of Soils 49:465-479. https://doi.org/10.1007/s00374-012-0737-7
Batool S, Iqbal A (2018). Phosphate solubilizing rhizobacteria as alternative of chemical fertilizer for growth and yield of Triticum aestivum (Var. Galaxy 2013). Saudi Journal of Biological Sciences 26:1400-1410. https://doi.org/10.1016/j.sjbs.2018.05.024
da Silva GJ, Rivadavea WR, de Lima JD, Monteiro PHR, da Silva FB (2021). Microbial enzymes and soil health. In: Mohamed HI, El-Beltagi HEDS, Abd-Elsalam KA (Eds). Plant growth-promoting microbes for sustainable biotic and abiotic stress management. Springer, Cham pp 133-155.
de Oliveira Mendes G, Moreira de Freitas AL, Liparini Pereira O, Ribeiro da Silva I, Bojkov Vassilev N, Dutra Costa M (2014). Mechanisms of phosphate solubilization by fungal isolates when exposed to different P sources. Annals of Microbiology 64:239-249. https://doi.org/10.1007/s13213-013-0656-3
De Zutter N, Ameye M, Bekaert B, Verwaeren J, De Gelder L, Audenaert K (2022). Uncovering new insights and misconceptions on the effectiveness of phosphate solubilizing rhizobacteria in plants: A meta-analysis. Frontiers in Plant Science 13:858804. https://doi.org/10.3389/fpls.2022.858804
Djuuna IAF, Prabawardani S, Massora M (2022). Population distribution of phosphate-solubilizing microorganisms in agricultural soil. Microbes and Environments 37(1):ME21041. https://doi.org/10.1264/jsme2.ME21041
El Habil-Addas F, Aarab S, Rfaki A, Laglaoui A, Bakkali M, Arakrak A (2017). Screening of phosphate solubilizing bacterial isolates for improving growth of wheat. European Journal of Biotechnology and Bioscience 2(6):7-11.
Glick BR (2012). Plant growth promoting bacteria: Mechanisms and applications. Scientifica 2012:963401. https://doi.org/10.6064/2012/963401
Hazarika SN, Saikia K, Borah A, Thakur D (2021). Prospecting endophytic bacteria endowed with plant growth promoting potential isolated from Camellia sinensis. Frontiers in Microbiology 12:738058. https://doi.org/10.3389/fmicb.2021.738058
Iyer B, Rajput MS, Rajkumar S (2017). Effect of succinate on phosphate solubilization in nitrogen fixing bacteria harbouring chick pea and their effect on plant growth. Microbiological Research 202:43-50. https://doi.org/10.1016/j.micres.2017.05.005
Jasnić SM, Miloš VB, Bagi F, Djordjevic V (2005). Pathogenicity of Fusarium species in soybean. Matica Srpska Proceedings for Natural Sciences 109:113-121. https://doi.org/10.2298/ZMSPN0519113J
Kadmiri IM, Chaouqui L, Azaroual SE, Sijilmassi B, Yaakoubi K, Wahby I (2018). Phosphate-solubilizing and auxin-producing rhizobacteria promote plant growth under saline conditions. Arabian Journal for Science and Engineering 43:3403-3415. https://doi.org/10.1007/s13369-017-3042-9
Kandil S, El Soda M (2015). Influence of freezing and freeze drying on intracellular enzymatic activity and autolytic properties of some lactic acid bacterial strains. Advances in Microbiology 5:371-382. http://dx.doi.org/10.4236/aim.2015.56039
Lanka S, Latha NL (2015). A short review on various screening methods to isolate potential lipase producers: Lipases-the present and future enzymes of biotech industry. International Journal of Biological Chemistry 9:207-219.
Lee JH, Anderson AJ, Kim YC (2022). Root-associated bacteria are biocontrol agents for multiple plant pests. Microorganisms 10:1053. https://doi.org/10.3390/microorganisms10051053
Lelapalli S, Baskar S, Jacob SM, Paranthaman S (2021). Characterization of phosphate solubilizing plant growth promoting rhizobacterium Lysinibacillus pakistanensis strain PCPSMR15 isolated from Oryza sativa. Current Research in Microbial Sciences 4(2):100080. https://doi.org/10.1016/j.crmicr.2021.100080
Li KS, Zeghbroeck JV, Liu Q, Zhang S (2021). Isolating and characterizing phosphorus solubilizing bacteria from rhizospheres of native plants grown in calcareous soils. Frontiers in Environmental Science 9:802563. https://doi.org/10.3389/fenvs.2021.802563
Mei C, Chretien RL, Amaradasa BS, He Y, Turner A, Lowman S (2021). Characterization of phosphate solubilizing bacterial endophytes and plant growth promotion in vitro and in greenhouse. Microorganisms 9(9):1935. https://doi.org/10.3390/microorganisms9091935
Migunova VD, Sasanelli N (2021). Bacteria as biocontrol tool against phytoparasitic nematodes. Plants 10(2):389. https://doi.org/10.3390/plants10020389
Mishra P, Mishra J, Dwivedi SK, Arora NK (2020). Microbial enzymes in biocontrol of phytopathogens. In: Arora N, Mishra J, Mishra V (Eds). Microbial enzymes: Roles and applications in industries. microorganisms for sustainability 11. Springer, Singapore pp. 259-285. https://doi.org/10.1007/978-981-15-1710-5_10
Neha VH, Visnuvinayagam S, Murthy LN, Jeyakumari A, Sivaraman GK, Prasad MM (2019). Characterization of vibriocin and prospect of co-culture method to overcome the diminishing antibacterial activity. International Journal of Current Microbiology and Applied Sciences 8(10):67-681. https://doi.org/10.20546/ijcmas.2019.810.076
Padder SA, Rather RA, Bhat SA, Shah MD, Baba TR, Mubarak NM (2022). Dynamics, phylogeny and phyto stimulating potential of chitinase synthesizing bacterial root endosymbiosiome of North Western Himalayan Brassica rapa L. Scientific Reports 12:6742. https://doi.org/10.1038/s41598-022-11030-0
Patel HK, Vyas RV, Shelat HN (2021). Selective enrichment method for isolation of efficient phosphate solubilizing bacteria from soil. Communications in Soil Science and Plant Analysis 53(12):1532-1541. https://doi.org/10.1080/00103624.2022.2055054
Pathak R, Paudel V, Shrestha A, Lamichhane J, Gauchan DP (2017). Isolation of phosphate solubilizing bacteria and their use for plant growth promotion in tomato seedling and plant. Kathmandu University Journal of Science, Engineering and Technology 13(II):61-70.
Rat A, Naranjo HD, Krigas N, Grigoriadou K, Maloupa E, Alonso AV, … Willems A (2021). Endophytic bacteria from the roots of the medicinal plant Alkanna tinctoria Tausch (Boraginaceae): Exploration of plant growth promoting properties and potential role in the production of plant secondary metabolites. Frontiers in Microbiology 12:633488. https://doi.org/10.3389/fmicb.2021.633488
Shahinur MR, Quadir QF, Rahman A, Asha MN, Chowdhury MAK (2014). Screening and characterization of phosphorus solubilizing bacteria and their effect on rice seedlings. Research in Agriculture Livestock and Fisheries 1(1):27-35. https://doi.org/10.3329/ralf.v1i1.22353
Suleman M, Yasmin S, Rasul M, Yahya M, Atta BM, Mirza MS (2018). Phosphate solubilizing bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat. PLoS One 13(9):e0204408. https://doi.org/10.1371/journal.pone.0204408
Sun M, Xiao T, Ning Z, Xiao E, Sun W (2015). Microbial community analysis in rice paddy soils irrigated by acid mine drainage contaminated water. Applied Microbiology and Biotechnology 9(9):2911-2922. https://doi.org/10.1007/s00253-014-6194-5
Vinod Babu S, Triven S, Reddy RS, Sathyanarayana J (2017). Screening of maize rhizosperic phosphate solubilizing isolates for plant growth promoting characteristics. International Journal of Current Microbiology and Applied Sciences 6(10):2090-2101. https://doi.org/10.20546/ijcmas.2017.610.249
Vyas P, Gulati A (2009). Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbiology 9:174. https://doi.org/10.1186/1471-2180-9-174
Walpola BC, Song J-S, Yoon M-H (2012). Assessment of plant growth promoting activities of phosphorus solubilizing bacteria. Korean Journal of Soil Science and Fertilizer 45(1):66-73. https://doi.org/10.7745/KJSSF.2012.45.1.066
Wasoontharawat M, Jatarasa R, Duangpaeng A (2017). Isolation and screening phosphoric solubilizing bacteria from organic anthill fertilizer and phosphorus release capacity. Sakon Nakhon Rajabhat University Journal of Science and Technology 9(2):454-464.
Yu H, Wu X, Zhang G, Zhou F, Harvey PR, Wang L, … Zhang X (2022). Identification of the phosphorus-solubilizing bacteria strain JP233 and its effects on soil phosphorus leaching loss and crop growth. Frontiers in Microbiology 13:892533. https://doi.org/10.3389/fmicb.2022.892533
Zhou J, Deng Y, Shen L, Wen C, Yan Q, Ning D, … Brown JH (2016). Temperature mediates continental-scale diversity of microbes in forest soils. Nature Communications 7(1):1-10. https://doi.org/10.1038/ncomms12083
How to Cite
Copyright (c) 2022 Hieu Q. NGUYEN, My NGUYEN, Nhu N. NGUYEN
This work is licensed under a Creative Commons Attribution 4.0 International License.
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.