In-vitro evaluation of antioxidant and antiradical potential of successive extracts, semi-purified fractions and biosynthesized silver nanoparticles of Rumex vesicarius
Keywords:biological activities; chemical constituents; Rumex vesicarius; silver nanoparticles; successive extracts
The aim of the present study was to assess in vitro the antiradical and antioxidant activities of successive extracts and semi-purified fractions from Rumex vesicarius L. In the present work, three extracts (n-Hexane, ethyl acetate and methanol) and 22 column fractions of methanolic extract (as promising extract) were evaluated against 2,2-diphenyl- 1-picrylhydrazyl (DPPH•) and 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) radical scavenging methods as antiradical and antioxidant activities compared with Butylated hydroxytoluene (BHT) as synthetic standard and silver nanoparticles of methanolic extract (Ag-NPs-Me), in addition to analysis of chemical constituents of extract and fraction using Gas chromatography–mass spectrometry (GC-MS). The obtained results revealed that, both methods go parallel showing that the concentration of extract and incubation time are dependent and proportional with phenolic compounds concentration. Absolute methanol extract recorded the highest antioxidant activity when compared with the other crude extracts with 79.3 and 78.8% against DPPH and ABTS respectively when compared with BHT as synthetic standard (89.4 and 89.9%) against DPPH and ABTS respectively. Calculation of the antiradical activity units showed the highest values of methanolic extract and its promising fraction (No. 12) after 300 seconds (5 minutes) comparing with antioxidant activity (30 min). Also, the antioxidant activity increased with synthetic Ag-NPs-Me when compared with methanolic extract by (IC50= 53.9 and 74.6 µg/ml respectively). Thus, the GC-MS analysis of successive extracts of R. vesicarius L showed a highly complex profile, containing approximately 24 different components. One pure compound was identified from fraction No. 12. The identified compound was l-(+)- ascorbic acid 2, 6-dihexadecanoate. The data also revealed presence of closely similar antioxidant activities in methanolic extract or its pure compounds with BHT when mixed at different proportions. From the obtained results it could be concluded that R. vesicarius methanolic extracts and fractions can be extensively used in the production of potential antioxidant, antiradical and AgNPs-Me for biomedical application on the consumer’s health.
Abd El-Maksoud AA, Abd El-Ghany IH, El-Beltagi HS, Anankanbil S, Banerijee C, Petersen SV, Guo Z (2018). Adding functionality to milk-based protein: preparation, and physicochemical characterization of β-lactoglobulinphenolic conjugates. Food Chemistry 241:281-289. https://doi.org10.1016/j.foodchem.2017.08.101
Abdel-Rahim EA, El-Beltagi HS (2010). Constituents of apple, parsley and lentil edible plants and their therapy treatments for blood picture as well as liver and kidney functions against lipidemic disease. Electronic Journal of Environmental, Agricultural and Food Chemistry 9(6):1117-1127.
Abdulla KA, Um CY, Gross MD, Bostick RM (2018). Circulating γ-tocopherol concentrations are inversely associated with antioxidant exposures and directly associated with systemic oxidative stress and inflammation in adults. The Journal of Nutrition 148(9):1453-1461. https://doi.org/10.1093/jn/nxy132
Afify AEMM, El-Beltagi HS (2011). Effect of insecticide cyanophos on liver function in adult male rats. Fresenius Environmental Bulletin 20(4a):1084-1088.
Afify AEMM, El-Beltagi HS,Aly AA, El-Ansary AE (2012). Antioxidant enzyme activities and lipid peroxidation as biomarker for potato tuber stored by two essential oils caraway and clove and its main component carvone and eugenol. Asian Pacific Journal of Tropical Biomedicine 2:S772-S780. https://doi.org/10.1016/S2221-1691(12)60312-8
Aboul-Enein AM, Shanab SM, Shalaby EA, Zahran MM, Lightfoot DA, El-Shemy HA (2014). Cytotoxic and antioxidant properties of active principals isolated from water hyacinth against four cancer cells lines. BMC Complementary and Alternative Medicine 14:397. https://doi.org/10.1186/1472-6882-14-397
Adewale OB, Egbeyemi KA, Onwuelu JO, Potts-Johnson SS, Anadozie SO, Fadaka AO, … Onasanya A (2020). Biological synthesis of gold and silver nanoparticles using leaf extracts of Crassocephalum rubens and their comparative in vitro antioxidant activities. Heliyon 6:e05501. https://doi.org/10.1016/j.heliyon.2020.e05501
Ahmeda A, Zangeneh A, Zangeneh MM (2020). Green synthesis and chemical characterization of gold nanoparticle synthesized using Camellia sinensis leaf aqueous extract for the treatment of acute myeloid leukemia in comparison to daunorubicin in a leukemic mouse model. Applied Organometallic Chemistry 34:e5290. https://doi.org/10.1002/aoc.5290
Al Aboody MS (2015). In vitro screening of phytochemical, antibacterial and antioxidant activities of Rumex vesicarius L. International Journal of Current Microbiology and Applied Sciences 4(9):884-893.
Al-Abd AM, Alamoudi AJ, Abdel-Naim AB, Neamatallah TA, Ashour OM (2017). Anti-angiogenic agents for the treatment of solid tumors: potential pathways, therapy and current strategies – a review. Journal of Advanced Research 8(6):591-605. https://doi.org/10.1016/j.jare.2017.06.006
Alberto MR, Canavosio MAR, Nadra MCM (2006). Antimicrobial effect of polyphenols from apple skins on human bacterial pathogens. Electronic Journal of Biotechnology 9:118-125.
Aly AA, Mohamed HI, Mansour MTM, Omar MR (2013). Suppression of powdery mildew on flax by foliar application of essential oils. Journal of Phytopathology 161:376-381. https://doi.org/10.1111/jph.12080
Akladious SA, Mohamed HI (2017). Physiological role of exogenous nitric oxide in improving performance, yield and some biochemical aspects of sunflower plant under zinc stress. Acta Biologica Hungarica 68(1):101-114. https://doi.org/10.1556/018.68.2017.1.9
Amira MA, El-Deeb K, Al-Otibi FO (2011). Assessment of antifungal activity of Rumex vesicarius L. and Ziziphus spina-christi (L.) Willd. extracts against two phytopathogenic fungi. African Journal of Microbiology Research 5(9):1001-1011. https://doi.org/10.5897/AJMR10.826
Ammar NM, Ayoub NA, El-Ahmady SH, Abou El-Kassem LT, Abou Zeid EM (2015). Phytochemical and cytotoxic studies of Rumex pictus Forssk. and Rumex vesicarius L. (Family Polygonaceae), growing in Egypt. European Journal of Medicinal Plants 10(3):1-13. https://doi.org/10.9734/EJMP/2015/19830
Ara I, Bukhari NA, Solaiman, D, Bakir MA (2012). Antimicrobial effect of local medicinal plant extracts in the Kingdom of Saudi Arabia and search for their metabolites by gas chromatography-mass spectrometric (GC-MS) analysis. Journal of Medicinal Plants Research 6(45):5688-5694.
Atteia BMR, El‐Kak AE, Lucchesi PA, Delafontane P (2009). Antioxidant activity of folic acid: From mechanism of action to clinical application. Experimental Biology 23(1):103.
Baker, CJ, Mock NM, Roberts DP, Deahl KL, Hapeman CJ, Schmidt WF, Kochansky J (2007). Interference by Mes [2-(4-morpholino) ethanesulfonic acid] and related buffers with phenolic oxidation by peroxidase. Free Radical Biology and Medicine 43(9):1322-1327. https://doi.org/10.1016/j.freeradbiomed.2007.07.020
Baskar AA, Al Numair KS, Gabriel Paulraj M, Alsaif MA, Muamar MA, Ignacimuthu S (2012). β-sitosterol prevents lipid peroxidation and improves antioxidant status and histoarchitecture in rats with 1,2-dimethylhydrazine-induced colon cancer. Journal of Medicinal Food 15(4):335-43. https://doi.org/10.1089/jmf.2011.1780
Begum SMFM, Priya S, Sundararajan R, Hemalatha S (2017). Novel anticancerous compounds from Sargassum wightii: In silico and in vitro approaches to test the antiproliferative efficacy. Journal of Advanced Pharmacy Education & Research 7(3):272-277.
Chahardoli A, Karimi N, Sadeghi F, Fattahi A (2018). Green approach for synthesis of gold nanoparticles from Nigella arvensis leaf extract and evaluation of their antibacterial, antioxidant, cytotoxicity and catalytic activities. Artificial Cells, Nanomedicine, and Biotechnology 46(3):579-588. https://doi.org/10.1080/21691401.2017.1332634
Chu W, Lim Y, Radhakrishnan AK, Lim P (2010). Protective effect of aqueous extract from Spirulina platensis against cell death induced by free radicals. BMC Complementary and Alternative Medicine 10:53. https://doi.org/10.1186/1472-6882-10-53
Dawi F, El-Beltagi HS, Abdel-Mobdy YE, Salah SM, Ghaly IS, Abdel-Rahim EA, ... Soliman AM (2021). Synergistic impact of the pomegranate peels and its nanoparticles against the infection of tobacco mosaic virus (TMV). Fresenius Environmental Bulletin 30(1):731-746.
Desai R, Mankad V, Gupta SK, Jha PK (2012). Size distribution of silver nanoparticles: UV-visible spectroscopic assessment. Nanoscience and Nanotechnology Letters 4:30-34. https://doi.org/10.1166/nnl.2012.1278
El-Beltagi HES (2011). Effect of roasting treatments on protein fraction profiles, some enzyme activities of Egyptian peanuts. International Journal of Food Sciences and Nutrition 62(5):453-456.
El-Beltagi HS, Ahmed SH, Namich AAM, Abdel-Sattar RR (2017) Effect of salicylic acid and potassium citrate on cotton plant under salt stress. Fresenius Environmental Bulletin 26(1a):1091-1100.
El-Beltagi HS, Mohamed HI, Safwat G, Megahed BMH, Gamal M (2018) Evaluation of some chemical constituents, antioxidant, antibacterial and anticancer activities of Beta vulgaris L. root. Fresenius Environmental Bulletin 27(9):6369-6378.
El-Beltagi HS, Mohamed HI, Elmelegy AA, Eldesoky SE, Safwat G (2019a). Phytochemical screening, antimicrobial, antioxidant, anticancer activities and nutritional values of cactus (Opuntia Ficus Indicia) pulp and peel. Fresenius Environmental Bulletin 28(2A):1534-1551.
El-Beltagi HS, Mohamed HI, Safwat G, Gamal M, Megahed BMH (2019b). Chemical composition and biological activity of Physalis peruviana L. Gesunde Pflanzen 71:113-122. https://doi.org/10.1007/s10343-019-00456-8
El-Beltagi HS, Mohamed HI, Sofy MR (2020a) Role of ascorbic acid, glutathione and proline applied as singly or in sequence combination in improving chickpea plant through physiological change and antioxidant defense under different levels of irrigation intervals. Molecules 25:1702. https://doi.org/10.3390/molecules25071702
El-Beltagi HS, Sofy MR, Aldaej MI, Mohamed HI (2020b) Silicon alleviates copper toxicity in flax plants by up-regulating antioxidant defense and secondary metabolites and decreasing oxidative damage. Sustainability 12:4732. https://doi.org/10.3390/su12114732
El-fayoumy EA, Shanab SM, Gaballa HS, Tantawy MA, Shalaby EA (2021). Evaluation of antioxidant and anticancer activity of crude extracts and different fractions of Chlorella vulgaris axenic culture grown under various concentrations of copper ions. BMC Complementary Medicine and Therapies 21:1-16. https://doi.org/10.1186/s12906-020-03194-x
Farooq M, Abutaha N, Mahboob M, Baabbad N, Almoutiri ND, Wadaan MAAM (2020). Investigating the antiangiogenic potential of Rumex vesicarius (humeidh), anticancer activity in cancer cell lines and assessment of developmental toxicity in zebrafish embryos. Saudi Journal of Biological Sciences 27:611-622. https://doi.org/10.1016/j.sjbs.2019.11.042
Gaber NB, El-Dahy SI, Shalaby EA (2021). Comparison of ABTS, DPPH, permanganate, and methylene blue assays for determining antioxidant potential of successive extracts from pomegranate and guava residues. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-01386-0.
Gandhi, MSA, Kumar VS, Li Q (2020). Synthesis of silver nanoparticles using Rumex crispus extract and evaluation of their antibacterial activities. Asia Communications and Photonics Conference 2020, paper M4A.5 https://doi.org/10.1364/ACPC.2020.M4A.5
Harborne JB (1973). Phytochemical methods: A guide to modern technique of plant analysis. Chapmann and Hall, London, pp 271-279.
Hariprasad P, Ramakrishnan N (2011). Phytochemical screening and pharmacognostical evaluation of Rumex vesicarius L. International Journal of Pharma Tech Research 3(2):1078-1082.
Hariprasad PS, Ramakrishnan N (2011). GC-MS analysis of Rumex vesicarius L. International Journal of Drug Development & Research 3(2):272-279.
Huo Y, Singh P, Kim YJ, Soshnikova V, Kang J, Markus J, … Yang DC (2018). Biological synthesis of gold and silver chloride nanoparticles by Glycyrrhiza uralensis and in vitro applications. Artificial Cells, Nanomedicine, and Biotechnology 46(2):303-312. https://doi.org/10.1080/21691401.2017.1307213
Huyut Z, Beydemir Ş, Gülçin İ (2017). Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. Biochemistry Research International 2017:7616791. https://doi.org/10.1155/2017/7616791
Ilya E, Kulikova L, Van der Eycken EV, Voskressensky L (2018). Recent advances in phthalan and coumaran chemistry. Chemistry Open 7(11):914-929. https://doi:10.1002/open.201800184.
Imran M, Raja MM, Basith JA (2011). Determination of total phenol, flavonoid and antioxidant activity of edible mushrooms Pleurotus florida and Pleurotus eous. International Food Research 18:574-577.
Jin X, Simeon NC, Palma J, Kim D, Ngabire D, Kim N-H, … Kim G-D (2018). Anticancer activity of Sasa borealis leaf extract-mediated gold nanoparticles AU -Patil, Maheshkumar Prakash. Artificial Cells, Nanomedicine, and Biotechnology 46(1):82-88.
Kaviarasan S, Naik GH, Gangabhagirathi R, Anuradha CV, Priyadarsini KI (2007). In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foeveem) seeds. Food Chemistry 103:31-37. https://doi.org/10.1016/j.foodchem.2006.05.064
Khan TH, Ganaie MA, Siddiqui AN, Alam, A, Ansari NM (2014). Antioxidant potential of Rumex vesicarius L.: in vitro approach. Asian Pacific Journal of Tropical Biomedicine 4(7):538-544. https://doi.org/10.12980/APJTB.4.2014C1168
Khan S, Richa, Kaur H, Jhamta R (2019). Evaluation of antioxidant potential and phytochemical characterization using GCMS analysis of bioactive compounds of Achillea filipendulina (L.) leaves. Journal of Pharmacognosy and Phytochemistry 8(3):258-265.
Khattak U, Ullah R, Khan S, Afriq S, Rauf A, Hassanien M (2019). Synthesis, characteristics and biological activities of silver nanoparticles from Euphorbia dracunculoides. EurAsian Journal of BioSciences 13(2):2249-2260.
Lakshmanan G, Sathiyaseelan A, Kalaichelvan PT, Murugesan K (2018). Plant-mediated synthesis of silver nanoparticles using fruit extract of Cleome viscosa L.: assessment of their antibacterial and anticancer activity. Karbala International Journal of Modern Science 4(1):61-68. https://doi.org/10.1016/j.kijoms.2017.10.007
Melichacova S, Timoracka M, Bystrick J, Vollmannova A, Cery I (2010). Relation of total antiradical activity and total polyphenol content of sweet cherries (Prunus avium L.) and tart cherries (Prunus cerasus L.). Acta Agriculturae Slovenica 95(1):21-28. https://doi.org/10.2478/v10014-010-0003-3
Nair R, Kalariya T, Chanda S (2005). Antibacterial activity of some selected Indian medicinal flora. Turkish Journal of Biology 29:41-47.
Ndikau M, Noah NM, Andala DM, Masika E (2017). Green synthesis and characterization of silver nanoparticles using Citrullus lanatus fruit rind extract. International Journal of Analytical Chemistry 8108504. https://doi.org/10.1155/2017/8108504.
Mohamed AA, El-Beltagi HS, Rashed MM (2009). Cadmium stress induced change in some hydrolytic enzymes, free radical formation and ultrastructural disorders in radish plant. Electronic Journal of Environmental, Agricultural and Food Chemistry 8(10):969-983.
Mohamed HI, El-Beltagi HS, Aly AA, Latif HH (2018a). The role of systemic and non systemic fungicides on the physiological and biochemical parameters in Gossypium hirsutum plant, implications for defense responses. Fresenius Environmental Bulletin 27(12):8585-8593.
Mohamed HI, Akladious SA, El-Beltagi HS (2018b). Mitigation the harmful effect of salt stress on physiological, biochemical and anatomical traits by foliar spray with trehalose on wheat cultivars. Fresenius Environmental Bulletin 27(10):7054-7065.
Nivas D, Gaikwad DK, Havan PD (2010). Antiradical activity of radically important Morinda pubescens fruits. International Journal of Pharma and Bio Sciences 1(3):1-4.
Panduraju T, Rao RS, Kumar SV (2009). A study on antimicrobial activity of Rumex vesicarius L. International Journal of Pharmacy and Technology 1:21-25.
Panja S, Choudhuri I, Khanra K, Pati B, Bhattacharyya N (2020). Biological and Photocatalytic activity of silver nanoparticle synthesized from Ehretia laevis Roxb. leaves extract. Nano Biomedicine and Engineering 12(1):104-113.
Prema R, Sekar DSS, Sekhar KBC, Jeevanandham S (2012). In vitro cytotoxicity study on combined plants extracts (Cissus quadrangularis and Aegle marmelos). European Journal of Experimental Biology 2:882-888.
Rao BN (2003). Bioactive phytochemicals in Indian foods and their potential in health promotion and disease prevention. Asia Pacific Journal of Clinical Nutrition 12:9-22.
Re R, Pellegrini RN, Proteggente A, Pannala A, Yang M, Rice- Evans C (1999). Antioxidant activity applying improved ABTS radical cation decolorization assay. Free Radical Biology and Medicinal 26:1231-1237. https://doi.org/10.1016/s0891-5849(98)00315-3
Rossenthaler L (1930). The chemical investigation of plants. Translated into English by Sudhamoy Ghosh from the Third German edition. Bell and Sons. Ltd London.
Salari S, Esmaeilzadeh Bahabadi S, Samzadeh-Kermani A, Yosefzaei F (2019). In-vitro evaluation of antioxidant and antibacterial potential of greensynthesized silver nanoparticles using Prosopis farcta fruit extract. Iranian Journal of Pharmaceutical Research 18(1):430-455.
Sayik A, Serguzel A, Yusufoglu AS, Acik L, Turker G, Aydin B, Arslan L (2017). DNA- binding, biological activities, and chemical composition of wild growing Epilobium angustifolium L. extracts from Canakkale, Turkey. Journal of Turkish Chemical Society 4(3):811-840.
Shalaby EA, Shanab SMM (2013). Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian Journal of Geo-Marine Sciences 42(5):556-564.
Shallan MA, El-Beltagi HS, Mona AM, Amera TM, Sohir NA (2010). Effect of amylose content and pre-germinated brown rice on serum blood glucose and lipids in experimental animal. Australian Journal of Basic and Applied Scieces 4(2):114-121.
Shanab SM, Mostafa SS, Shalaby EA, Mahmoud GI (2012). Aqueous extracts of microalgae exhibit antioxidant and anticancer activities. Asian Pacific Journal of Tropical Medicine 2:608-615. https://doi.org/10.1016/S2221-1691(12)60106-3
Sheela D, Uthayakumaria F (2013). GC-MS analysis of bioactive constituents from coastal sand dune taxone-Sesuivum portulacastrum. Bioscience Discovery 4(1):47-53.
Sroka Z (2006). The screening analysis of antiradical activity of some plant extracts. Postepy Higieny i Medycyny Doswiadczalnej 60: 563-570.
Stevic T, Savikin K, Ristic M, Zdunic G, Jan kovic T, Krivokuca D, Vulic T (2010). Composition and antimicrobial activity of the essential oil of the leaves of black currant (Ribes nigrum L.) cultivar Cacanska crna. Journal of Serbian Chemical Society 75(1):35-43. https://doi.org/10.2298/JSC1001035S
Sudha A, Jeyakanthan J, Srinivasan P (2017). Green synthesis of silver nanoparticles using Lippia nodiflora aerial extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Resource-Efficient Technologies 3(4):506-515. https://doi.org/10.1016/j.reffit.2017.07.002
Tirzitis G, Bartosz G (2010). Determination of antiradical and antioxidant activity: basic principles and new insights, Acta Biochemica Polonica 75(1):139-142.
Trease GE, Evans WC (1983). The text book of pharmacognosy. (12th Edn.,) Balliere, Tindall, London, pp 57-59.
Vanaja M, Shanmugam R, Paulkumar K, Gnanajobitha G (2013). Kinetic study on green synthesis of silver nanoparticles using Coleus aromaticus leaf extract. Advances in Applied Science Research 4(3):50-55.
Wang J, Liu H, Zhao J, Gao H, Zhou L, Liu Z, Chen Y, Sui P (2010). Antimicrobial and antioxidant activities of the root bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde. Molecules 15(8):5807-5817. https://doi.org/10.3390/molecules15085807
Wen XB, Miao F, Zhou L, Zhang M, He QL (2012). In vitro antioxidant activity of Parnassia wightiana W. extracts. Chinese Journal of Natural Medicines 10(3):190-195. https://doi.org/10.3724/SP.J.1009.2012.00190
Wondrak GT, Cabello CM, Villeneuve NF, Zhang S, Ley Y, Li S, … Zhang DD (2008). Cinnamoyl-based Nrf2-activators targeting human skin cell photo-oxidative stress. Free Radical Biology and Medicine 45(4):385-395. https://doi.org/10.1016/j.freeradbiomed.2008.04.023
Yen GC, Chen HY (1995). Antioxidant activity of various tea extracts in relation to their anti-mutagenicity. Journal of Agriculture and Food Chemistry 43:27-37. https://doi.org/10.1021/jf00049a007
Yousaf H, Mehmood A, Ahmed KS, Raffi M (2020). Green synthesis of silver nanoparticles and their applications as an alternative antibacterial and antioxidant agent. Materials Science and Engineering: C 112:110901. https://doi.org/10.1016/j.msec.2020.110901
Yugaya YA, Usoltsevab RV, Silantevc VE, Egorovaad AE, Karabtsove AA, Kumeikodf VV, … Shkryl YN (2020). Synthesis of bioactive silver nanoparticles using alginate, fucoidan and laminaran from brown algae as a reducing and stabilizing agent. Carbohydrate Polymers 245:116547. https://doi.org/10.1016/j.carbpol.2020.116547
Zabot GP, Carvalhal GF, Marroni NP, Licks F, Hartmann RM, da Silva VD, Fillmann HS. (2017). Glutamine prevents oxidative stress in a model of portal hypertension. World Journal of Gastroenterology 23(25):4529-4537. https://doi.org/10.3748/wjg.v23.i25.4529
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